{"id":5412,"date":"2026-08-07T06:55:33","date_gmt":"2026-08-06T21:55:33","guid":{"rendered":"https:\/\/www.aem.imr.tohoku.ac.jp\/?p=5412"},"modified":"2026-08-07T06:55:33","modified_gmt":"2026-08-06T21:55:33","slug":"jem-2100plus%ef%bc%9astem-bf-df","status":"publish","type":"post","link":"https:\/\/www.aem.imr.tohoku.ac.jp\/en\/jem-2100plus%ef%bc%9astem-bf-df\/","title":{"rendered":"JEM-2100Plus: Stem-Bf\/Df"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><em>Note: This page was translated by AI from the Japanese original. In case of any discrepancy, the Japanese version shall prevail.<\/em><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><em>Original post date (Japanese version): June 22, 2024<\/em><\/p>\n\n\n\n<p class=\"has-background wp-block-paragraph\">&#x1f4a1; This page is a quick manual for JEM-2100plus.\nContents are subject to change.\nPlease receive training before actually using the equipment.<\/p>\n\n\n<h3 class=\"wp-block-heading\">STEM-BF<\/h3>\n\n\n<ol class=\"wp-block-list\">\n<li><span><strong>Display a normal image in TEM mode.<\/strong><\/span>\n<ul class=\"wp-block-list\">\n<li>Objective aperture and selected field aperture are open.<\/li>\n<\/ul>\n<\/li>\n\n\n<li><span><strong>Switch to SCAN(STEM) mode<\/strong><\/span>\n<ul class=\"wp-block-list\">\n<li>[TC: TEM System Task Bar : Illumination System ] Click the [SCAN] button<\/li>\n<\/ul>\n<\/li>\n\n\n<li><span><strong>Insert the bright field image detector (on the right side of the lens barrel) into the lens barrel.<\/strong><\/span><\/li>\n\n\n<li><span><strong>Cover the observation window.<\/strong><\/span><\/li>\n\n\n<li><span><strong>Operation image display<\/strong><\/span>\n<ol class=\"wp-block-list\">\n<li>[TC: TEM System Task Bar : Image Forming System: Image Selector : BF ]\u3000BF detector selection<\/li>\n\n\n<li>[TC: TEM System Task Bar : Image Forming System: Camera Length ] Camera length setting\n<ul class=\"wp-block-list\">\n<li>Increasing the camera length increases contrast<\/li>\n<\/ul>\n<\/li>\n\n\n<li>[TC:STEM Image Viewer : Preset Mode : Search Mode] \u30b9\u30ad\u30e3\u30f3\u901f\u5ea6\u9078\u629e\uff08\u9ad8\u901f\uff09<\/li>\n<\/ol>\n<\/li>\n\n\n<li><span><strong>Brightness\/contrast adjustment<\/strong><\/span>\n<ol class=\"wp-block-list\">\n<li>(If necessary) [TC: Top menu: View: Histgram] Histogram display<\/li>\n\n\n<li>[TC: STEM Image Viewer : Contrast ] Contrast adjustment<\/li>\n\n\n<li>[TC: STEM Image Viewer : Brightness ] Brightness adjustment<\/li>\n<\/ol>\n\n\n<p class=\"has-background wp-block-paragraph\">&#x1f4a1; Automatic adjustment<\/p>\n<\/li>\n\n\n<li><span><strong>height adjustment<\/strong><\/span>\n<ol class=\"wp-block-list\">\n<li>[PANEL-R: STD FOCUS] Reference current call<\/li>\n\n\n<li>[PANEL-R: Z\u25b3\u25bd] Sample height (Z) adjustment: Obtain the sharpest image.\n<ol class=\"wp-block-list\">\n<li>If it is difficult to match, lower the magnification.<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<\/li>\n\n\n<li><span><strong>Focusing\/stigma correction<\/strong><\/span>\n<ol class=\"wp-block-list\">\n<li>[PANEL-R:OBJ Focus] Focusing<\/li>\n\n\n<li>[PANEL-L: DEF\/STIG: COND STIG] Focusing lens astigmatism adjustment mode selection<\/li>\n\n\n<li>[PANEL-L\/R: DEF\/STIG-X\/Y] Adjust to minimize image flow<\/li>\n\n\n<li>[TC:STEM Image Viewer : Preset Mode : Focus Mode] Scan speed selection (for focusing)<\/li>\n\n\n<li>Repeat a-c to get the sharpest image<\/li>\n<\/ol>\n<\/li>\n\n\n<li><span><strong>photograph<\/strong><\/span>\n<ol class=\"wp-block-list\">\n<li>[TC:STEM Image Viewer : Preset Mode : Photo Mode] Scan speed selection (for shooting: low speed)<\/li>\n\n\n<li>[TC:STEM Image Viewer ] Capture Single<\/li>\n<\/ol>\n<\/li>\n\n\n<li><span><strong>Exit (switch to TEM mode)<\/strong><\/span>\n<ol class=\"wp-block-list\">\n<li>[TC: TEM System Task Bar : Illumination System ] Click the [TEM] button<\/li>\n\n\n<li>pull out the detector<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n\n\n<h3 class=\"wp-block-heading\">STEM-DF<\/h3>\n\n\n<ol class=\"wp-block-list\">\n<li><span><strong>Display a normal image in TEM mode.<\/strong><\/span>\n<ul class=\"wp-block-list\">\n<li>Objective aperture and selected field aperture are open.<\/li>\n<\/ul>\n<\/li>\n\n\n<li><span><strong>Switch to SCAN(STEM) mode<\/strong><\/span>\n<ol class=\"wp-block-list\">\n<li>[TC: TEM System Task Bar : Illumination System] Click the [SCAN] button<\/li>\n\n\n<li>[TC: TEM System Task Bar : Image Forming System: Image Selector : DF ] DF detector selection<\/li>\n\n\n<li>[TC: TEM System Task Bar : Image Forming System: Camera Length ] Camera length setting (10 or 20cm)<\/li>\n<\/ol>\n<\/li>\n\n\n<li><span><strong>Ronchigram adjustment<\/strong><\/span>\n<ol class=\"wp-block-list\">\n<li><span><strong>screen work<\/strong><\/span>\n<ol class=\"wp-block-list\">\n<li>[TC: Image Forming System: Ronchigram Mode: On ] Ronchigram mode On (SCAN stop)<\/li>\n\n\n<li>[CLA#1(\u03c6150um)] Insert centering\n<p class=\"has-background wp-block-paragraph\">&#x1f4a1; Align the center of the aperture with the Ronchigram center<\/p>\n<\/li>\n<\/ol>\n<\/li>\n\n\n<li><span><strong>camera work<\/strong><\/span>\n<ol class=\"wp-block-list\">\n<li>[GMS : TEM Imaging : View] (Exposure : 0.1sec) \u30ab\u30e1\u30e9\u633f\u5165\u30fb\u8868\u793a<\/li>\n\n\n<li>[PANEL R : F4] Screen open<\/li>\n\n\n<li>Ronchigram Centering\n<ol class=\"wp-block-list\">\n<li>[PANEL-L: DEF\/STIG: PLA] Projection lens alignment mode selection<\/li>\n\n\n<li>[PANEL-L\/R: DEF\/STIG-X\/Y] Centering the transmitted wave to the center of the fluorescent screen<\/li>\n<\/ol>\n\n\n<p class=\"has-background wp-block-paragraph\">&#x1f4a1; Align the center of Ronchigram with the center of the screen.<\/p>\n<\/li>\n\n\n<li>[CLA#3 (\u03c650um)] Insertion centering\n<p class=\"has-background wp-block-paragraph\">&#x1f4a1; Align the center of Ronchigram and the center of CLA with the center of the screen.<\/p>\n<\/li>\n\n\n<li>[TC: Image Forming System: Ronchigram Mode : Off ] Ronchigram mode On (SCAN start)<\/li>\n\n\n<li>[GMS : TEM Imaging : View] Camera stop<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<\/li>\n\n\n<li><span><strong>Cover the observation window.<\/strong><\/span><\/li>\n\n\n<li><span><strong>Insert the dark field image detector (left side of the lens barrel) into the lens barrel.<\/strong><\/span><\/li>\n\n\n<li><span><strong>Operation image display<\/strong><\/span>\n<ol class=\"wp-block-list\">\n<li>[TC: TEM System Task Bar : Image Forming System: Camera Length ] Camera length setting<\/li>\n\n\n<li>[TC:STEM Image Viewer : Preset Mode : Search Mode] Scan speed selection (high speed)<\/li>\n<\/ol>\n<\/li>\n\n\n<li><span><strong>Brightness\/contrast adjustment<\/strong><\/span>\n<ol class=\"wp-block-list\">\n<li>(If necessary) [TC: Top menu: View: Histgram] Histogram display<\/li>\n\n\n<li>[TC: STEM Image Viewer : Contrast ] Contrast adjustment<\/li>\n\n\n<li>[TC: STEM Image Viewer : Brightness ] Brightness adjustment<\/li>\n<\/ol>\n\n\n<p class=\"has-background wp-block-paragraph\">&#x1f4a1; Automatic adjustment<\/p>\n<\/li>\n\n\n<li><span><strong>height adjustment<\/strong><\/span>\n<ol class=\"wp-block-list\">\n<li>[PANEL-R: STD FOCUS] Reference current call<\/li>\n\n\n<li>[PANEL-R: Z\u25b3\u25bd] Sample height (Z) adjustment: Obtain the sharpest image.\n<ol class=\"wp-block-list\">\n<li>If it is difficult to match, lower the magnification.<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<\/li>\n\n\n<li><span><strong>Focusing\/stigma correction<\/strong><\/span>\n<ol class=\"wp-block-list\">\n<li>[PANEL-R:OBJ Focus] Focusing<\/li>\n\n\n<li>[PANEL-L: DEF\/STIG: COND STIG] Focusing lens astigmatism adjustment mode selection<\/li>\n\n\n<li>[PANEL-L\/R: DEF\/STIG-X\/Y] Adjust to minimize image flow<\/li>\n\n\n<li>[TC:STEM Image Viewer : Preset Mode : Focus Mode] Scan speed selection (for focusing)<\/li>\n\n\n<li>Repeat a-c to get the sharpest image<\/li>\n<\/ol>\n<\/li>\n\n\n<li><span><strong>photograph<\/strong><\/span>\n<ol class=\"wp-block-list\">\n<li>[TC:STEM Image Viewer : Preset Mode : Photo Mode] Scan speed selection (for shooting: low speed)<\/li>\n\n\n<li>[TC:STEM Image Viewer ] Capture Single<\/li>\n<\/ol>\n<\/li>\n\n\n<li><span><strong>Exit (switch to TEM mode)<\/strong><\/span>\n<ol class=\"wp-block-list\">\n<li>[TC: TEM System Task Bar : Illumination System ]  [TEM]\u30dc\u30bf\u30f3\u30af\u30ea\u30c3\u30af <\/li>\n\n\n<li>pull out the detector<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n\n\n\n\n<p class=\"wp-block-paragraph\"><em>*This post was created by an AI agent.<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Note: This page was translated by AI from the Japanese original. In case of any discrepancy, the Japanese version shall prevail. Original post date (Japanese version): June 22, 2024 &#x1f4a1; This page is a quick manual for JEM-2100plus. Contents are subject to change. Please receive training before actually using the equipment. STEM-BF STEM-DF *This post <a href=\"https:\/\/www.aem.imr.tohoku.ac.jp\/en\/jem-2100plus%ef%bc%9astem-bf-df\/\" class=\"more-link\">&#8230;<span class=\"screen-reader-text\">  JEM-2100Plus: Stem-Bf\/Df<\/span><\/a><\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_locale":"en_US","_original_post":"https:\/\/www.aem.imr.tohoku.ac.jp\/?p=3224","footnotes":"","jetpack_post_was_ever_published":false},"categories":[1],"tags":[],"class_list":["post-5412","post","type-post","status-publish","format-standard","hentry","category-uncategorized","en-US"],"jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/posts\/5412","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/comments?post=5412"}],"version-history":[{"count":4,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/posts\/5412\/revisions"}],"predecessor-version":[{"id":5788,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/posts\/5412\/revisions\/5788"}],"wp:attachment":[{"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/media?parent=5412"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/categories?post=5412"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/tags?post=5412"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}