{"id":5443,"date":"2026-08-07T06:55:34","date_gmt":"2026-08-06T21:55:34","guid":{"rendered":"https:\/\/www.aem.imr.tohoku.ac.jp\/?p=5443"},"modified":"2026-08-07T06:55:34","modified_gmt":"2026-08-06T21:55:34","slug":"jem-2100plus%ef%bc%9a%e5%83%8f%e8%a6%b3%e5%af%9f%ef%bc%88%e5%9f%ba%e6%9c%ac%ef%bc%89","status":"publish","type":"post","link":"https:\/\/www.aem.imr.tohoku.ac.jp\/en\/jem-2100plus%ef%bc%9a%e5%83%8f%e8%a6%b3%e5%af%9f%ef%bc%88%e5%9f%ba%e6%9c%ac%ef%bc%89\/","title":{"rendered":"JEM-2100Plus: Image Observation (Basic)"},"content":{"rendered":"\n

Note: This page was translated by AI from the Japanese original. In case of any discrepancy, the Japanese version shall prevail.<\/em><\/p>\n\n\n\n

Original post date (Japanese version): June 22, 2024<\/em><\/p>\n\n\n\n

💡 \u672c\u30da\u30fc\u30b8\u306fJEM-2100plus\u306e\u30af\u30a4\u30c3\u30af\u30de\u30cb\u30e5\u30a2\u30eb\u3067\u3059\u3002\n\u5185\u5bb9\u306f\u5909\u66f4\u3055\u308c\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\n\u5b9f\u969b\u306b\u88c5\u7f6e\u3092\u4f7f\u7528\u3059\u308b\u5834\u5408\u306f\u3001\u4e8b\u524d\u306b\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u3092\u53d7\u3051\u3066\u4e0b\u3055\u3044\u3002<\/p>\n\n\n

    \n
  1. [PANEL-R : Function : MAG1] \u50cf\u89b3\u5bdf\u30e2\u30fc\u30c9\u3078<\/strong><\/span>\n
      \n
    1. [PANEL-L : APERTURE CONTROL :OL(OPEN)] Objective aperture: OPEN<\/li>\n\n\n
    2. [HCA] High contrast objective aperture: OPEN<\/li>\n\n\n
    3. [SA] Selected field aperture: OPEN<\/li>\n<\/ol>\n<\/li>\n\n\n
    4. [PANEL-R : STD FOCUS]\u3000Objective lens reference current call<\/strong><\/span>\n

      💡 STF FOCUS can be done frequently.<\/p>\n<\/li>\n\n\n

    5. Probe settings<\/strong><\/span>\n
        \n
      1. [PANEL-L: PROBE CONTROL: TEM] TEM mode<\/li>\n\n\n
      2. [PANEL-L : PROBE CONTROL : SPOT SIZE] SPOT SIZE setting (normal: 2)<\/li>\n\n\n
      3. [PANEL-L : PROBE CONTROL : \u03b1SELECTOR] Convergence angle (irradiation range) setting (normal: 2)<\/li>\n\n\n
      4. [CLA] Condenser aperture selection\/centering (normal: 2)<\/li>\n<\/ol>\n<\/li>\n\n\n
      5. [PANEL-R : MAG\/CAM L] Magnification setting<\/strong><\/span>(Usually: from about 10 to 50K)<\/li>\n\n\n
      6. beam centering<\/strong><\/span>\n
          \n
        1. [PANEL-L: BRIGHTNESS] Focus the beam on the fluorescent screen. (crossover)<\/li>\n\n\n
        2. [PANEL-L : DEF\/STIG : BRIGHT TILT] \u660e\u8996\u91ce\u7167\u5c04\u30e2\u30fc\u30c9\u9078\u629e<\/li>\n\n\n
        3. [PANEL-L\/R: SHIFT-X\/Y] Centering with Beam Shift<\/li>\n<\/ol>\n<\/li>\n\n\n
        4. Condenser lens astigmatism adjustment<\/strong><\/span>\n
            \n
          1. [PANEL-L: DEF\/STIG: COND STIG] Condenser lens astigmatism adjustment mode selection<\/li>\n\n\n
          2. [PANEL-L\/R: DEF\/STIG-X\/Y] (Cond STIG) Make the spot shape closer to a perfect circle.<\/li>\n<\/ol>\n<\/li>\n\n\n
          3. Brightness adjustment<\/strong><\/span>\n
              \n
            1. [PANEL-L: BRIGHTNESS] Brightness for easy observation: beam spread to the same extent as the screen<\/li>\n<\/ol>\n<\/li>\n\n\n
            2. Searching for field of view<\/strong><\/span>\n
                \n
              1. [TB] Move the observation target to the center of the field of view using the trackball<\/li>\n<\/ol>\n<\/li>\n\n\n
              2. Focusing (coarse)<\/strong><\/span>\n
                  \n
                1. [PANEL-R : STD FOCUS]\u3000\u57fa\u6e96\u96fb\u6d41\u547c\u3073\u51fa\u3057<\/li>\n\n\n
                2. [PANEL-R: Z\u25b3\u25bd] Sample height (Z) adjustment: Lowest contrast<\/li>\n<\/ol>\n<\/li>\n\n\n
                3. Objective lens astigmatism correction<\/strong><\/span>\n
                    \n
                  1. Medium double\n
                      \n
                    1. Observing the vicinity of the membrane hole (x20K ~)\n

                      Center the beam and adjust the brightness for easy observation.<\/p>\n<\/li>\n\n\n

                    2. [PANEL-L: DEF\/STIG: OBJ STIG] Objective lens astigmatism adjustment mode selection<\/li>\n\n\n
                    3. [PANEL-R: OBJ FOCUS] Display Fresnel fringes with hyperfocus<\/li>\n\n\n
                    4. [PANEL-L\/R: DEF\/STIG-X\/Y] Adjust the Fresnel fringe to be isotropic<\/li>\n<\/ol>\n<\/li>\n\n\n
                    5. High magnification\n
                        \n
                      1. \u975e\u6676\u8cea\u9818\u57df\uff08\u652f\u6301\u819c\u306a\u3069\uff09\u3092\u89b3\u5bdf\uff08x100K\uff5e\uff09\n

                        Center the beam and adjust the brightness for easy observation.<\/p>\n<\/li>\n\n\n

                      2. [PANEL-L: DEF\/STIG: OBJ STIG] Objective lens astigmatism adjustment mode selection<\/li>\n\n\n
                      3. [PANEL-R : OBJ FOCUS] \uff06[PANEL-L\/R: DEF\/STIG-X\/Y]\n
                          \n
                        1. Confirm that the image flows due to hyperfocal <> underfocal<\/li>\n\n\n
                        2. Adjust to minimize image flow<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<\/li>\n\n\n
                        3. Insert objective aperture (if necessary)<\/strong><\/span>\n
                            \n
                          1. (Simple)\n
                              \n
                            1. [OLA\/HCA] Objective aperture (in-gap orhigh contrast<\/strong>) insert<\/li>\n\n\n
                            2. [OLA\/HCA] Centering of objective aperture<\/li>\n<\/ol>\n<\/li>\n\n\n
                            3. \uff08\u63a8\u5968\uff09\n
                                \n
                              1. [SA] Inserting and centering the selected area diaphragm<\/li>\n\n\n
                              2. [PANEL-R : Function : DIFF] To diffraction mode: Electron diffraction display<\/li>\n\n\n
                              3. [OLA\/HCA] Objective aperture (in gap<\/strong>or high contrast) and select transmitted wave.<\/li>\n\n\n
                              4. [PANEL-R : Function : MAG1] Return to image observation mode<\/li>\n\n\n
                              5. [SA] Pull out the selected area diaphragm<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<\/li>\n\n\n
                              6. Focusing (precision)<\/strong><\/span>\n
                                  \n
                                1. [PANEL-R: OBJ FOCUS] Adjust to a focus that is easy to see near the positive focus\n
                                    \n
                                  1. \u6b63\u7126\u70b9\uff08\u30b8\u30e3\u30b9\u30c8\u30d5\u30a9\u30fc\u30ab\u30b9\uff09\uff1a\u819c\u5b54\u306e\u8f2a\u90ed\u306e\u30b3\u30f3\u30c8\u30e9\u30b9\u30c8\u6700\u5c0f<\/li>\n\n\n
                                  2. Hyperfocal (overfocus): Focus clockwise from positive focus<\/li>\n\n\n
                                  3. Underfocus: Move the focus from positive focus to counterclockwise.<\/li>\n<\/ol>\n\n\n

                                    💡 Samples with low contrast will be unclear at positive focus, so set the focus to be slightly underfocused.<\/p>\n<\/li>\n<\/ol>\n<\/li>\n\n\n

                                  4. (photograph)<\/span>\n
                                      \n
                                    1. [Screen] Lower the magnification for CCD camera photography.<\/li>\n\n\n
                                    2. [Screen] Adjust brightness<\/li>\n\n\n
                                    3. Display the image with a CCD camera and adjust focus and brightness<\/li>\n\n\n
                                    4. take a photo<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n\n\n\n\n

                                      *This post was created by an AI agent.<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"

                                      Note: This page was translated by AI from the Japanese original. In case of any discrepancy, the Japanese version shall prevail. Original post date (Japanese version): June 22, 2024 💡 \u672c\u30da\u30fc\u30b8\u306fJEM-2100plus\u306e\u30af\u30a4\u30c3\u30af\u30de\u30cb\u30e5\u30a2\u30eb\u3067\u3059\u3002 \u5185\u5bb9\u306f\u5909\u66f4\u3055\u308c\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002 \u5b9f\u969b\u306b\u88c5\u7f6e\u3092\u4f7f\u7528\u3059\u308b\u5834\u5408\u306f\u3001\u4e8b\u524d\u306b\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u3092\u53d7\u3051\u3066\u4e0b\u3055\u3044\u3002 *This post was created by an AI agent.<\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_locale":"en_US","_original_post":"https:\/\/www.aem.imr.tohoku.ac.jp\/?p=3210","footnotes":"","jetpack_post_was_ever_published":false},"categories":[1],"tags":[],"class_list":["post-5443","post","type-post","status-publish","format-standard","hentry","category-uncategorized","en-US"],"jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/posts\/5443","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/comments?post=5443"}],"version-history":[{"count":4,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/posts\/5443\/revisions"}],"predecessor-version":[{"id":5797,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/posts\/5443\/revisions\/5797"}],"wp:attachment":[{"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/media?parent=5443"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/categories?post=5443"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/tags?post=5443"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}