{"id":5449,"date":"2026-08-07T06:55:35","date_gmt":"2026-08-06T21:55:35","guid":{"rendered":"https:\/\/www.aem.imr.tohoku.ac.jp\/?p=5449"},"modified":"2026-08-07T06:55:35","modified_gmt":"2026-08-06T21:55:35","slug":"jem-2100plus%ef%bc%9a%e3%83%95%e3%82%a9%e3%83%bc%e3%82%ab%e3%82%b9%e5%90%88%e3%82%8f%e3%81%9b","status":"publish","type":"post","link":"https:\/\/www.aem.imr.tohoku.ac.jp\/en\/jem-2100plus%ef%bc%9a%e3%83%95%e3%82%a9%e3%83%bc%e3%82%ab%e3%82%b9%e5%90%88%e3%82%8f%e3%81%9b\/","title":{"rendered":"JEM-2100plus: Focus Alignment"},"content":{"rendered":"\n

Note: This page was translated by AI from the Japanese original. In case of any discrepancy, the Japanese version shall prevail.<\/em><\/p>\n\n\n\n

Original post date (Japanese version): June 22, 2024<\/em><\/p>\n\n\n\n

TEM: Image Mode<\/h3>\n\n\n

💡 This page is a quick manual for the JEM-2100plus.\nThe contents are subject to change.\nPlease receive training in advance before actually using the equipment.<\/p>\n\n\n

    \n
  1. Focusing (Coarse)<\/strong><\/span>\n
      \n
    1. [PANEL-R : STD FOCUS] Recall objective lens reference current<\/li>\n\n\n
    2. [PANEL-R : Z\u25b3\u25bd] Specimen height (Z) adjustment: Lower the contrast to the minimum<\/li>\n<\/ol>\n<\/li>\n\n\n\n
    3. Focusing (Fine)<\/strong><\/span>\n
        \n
      1. [PANEL-R : OBJ FOCUS] Adjust to an easy-to-see focus near the true focus\n
          \n
        1. True focus (Just focus): Minimum contrast of the outline of the hole in the film<\/li>\n\n\n
        2. Overfocus: Turn Focus clockwise from true focus<\/li>\n\n\n
        3. Underfocus: Turn Focus counterclockwise from true focus<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n\n\n

          💡 Low-contrast specimens will be unclear at true focus, so set to a slight underfocus.<\/p>\n<\/li>\n\n\n\n

        4. Focusing using Wobbler<\/strong>\n
            \n
          1. Complete focusing (coarse) in advance.<\/strong><\/span><\/li>\n\n\n
          2. [PANEL-R : IMAGEWOBBLER : IMAGE X or Y] Turn image wobbler On<\/li>\n\n\n
          3. Open the objective aperture. (Or set it to a large size)<\/li>\n\n\n
          4. If out of focus, the image will appear double.\n
              \n
            1. [PANEL-R : OBJ FOCUS] Adjust so that the image overlaps as one<\/li>\n<\/ol>\n\n\n

              💡 If the image does not overlap, correct the astigmatism of the objective lens<\/p>\n<\/li>\n\n\n

            2. [PANEL-R : IMAGEWOBBLER : IMAGE X or Y] Turn image wobbler Off<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n\n\n

              TEM: DIFF Mode<\/h3>\n\n\n

              (Reference) JEM-2100plus: Electron Diffraction (Basics)<\/p>\n\n\n\n

              *This post was created by an AI agent.<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"

              Note: This page was translated by AI from the Japanese original. In case of any discrepancy, the Japanese version shall prevail. Original post date (Japanese version): June 22, 2024 TEM: Image Mode 💡 This page is a quick manual for the JEM-2100plus. The contents are subject to change. Please receive training in advance before actually … JEM-2100plus: Focus Alignment<\/span><\/a><\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_locale":"en_US","_original_post":"https:\/\/www.aem.imr.tohoku.ac.jp\/?p=3205","footnotes":"","jetpack_post_was_ever_published":false},"categories":[1],"tags":[],"class_list":["post-5449","post","type-post","status-publish","format-standard","hentry","category-uncategorized","en-US"],"jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/posts\/5449","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/comments?post=5449"}],"version-history":[{"count":4,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/posts\/5449\/revisions"}],"predecessor-version":[{"id":5757,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/posts\/5449\/revisions\/5757"}],"wp:attachment":[{"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/media?parent=5449"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/categories?post=5449"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/tags?post=5449"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}