{"id":5450,"date":"2026-08-07T06:55:35","date_gmt":"2026-08-06T21:55:35","guid":{"rendered":"https:\/\/www.aem.imr.tohoku.ac.jp\/?p=5450"},"modified":"2026-08-07T06:55:35","modified_gmt":"2026-08-06T21:55:35","slug":"jem-2100plus%ef%bc%9a%e7%85%a7%e5%b0%84%e7%b3%bb%e8%aa%bf%e6%95%b4","status":"publish","type":"post","link":"https:\/\/www.aem.imr.tohoku.ac.jp\/en\/jem-2100plus%ef%bc%9a%e7%85%a7%e5%b0%84%e7%b3%bb%e8%aa%bf%e6%95%b4\/","title":{"rendered":"JEM-2100plus: Illumination System Alignment"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><em>Note: This page was translated by AI from the Japanese original. In case of any discrepancy, the Japanese version shall prevail.<\/em><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><em>Original post date (Japanese version): June 22, 2024<\/em><\/p>\n\n\n\n<p class=\"has-background wp-block-paragraph\">&#x1f4a1; This page is a quick manual for the JEM-2100plus.\nThe contents are subject to change.\nPlease receive training in advance before actually using the equipment.<\/p>\n\n\n<p class=\"has-background wp-block-paragraph\">&#x1f4a1; Basic axis alignments are assumed to have been completed by the administrator.<\/p>\n\n\n<h3 class=\"wp-block-heading\">BRIGHTNESS Adjustment<\/h3>\n\n\n<ol class=\"wp-block-list\">\n<li>[PANEL-L : BRIGHTNESS] Brightness adjustment\n<ol class=\"wp-block-list\">\n<li>Turn it fully clockwise once (maximum excitation\/alarm sounds), then return it counterclockwise (converge).\n<ol class=\"wp-block-list\">\n<li>Clockwise: CL3 excitation strengthens: Focal length shortens\n<ol class=\"wp-block-list\">\n<li>When turned fully, the beam diverges (becomes darker).<\/li>\n<\/ol>\n<\/li>\n\n\n<li>Counterclockwise: CL3 excitation weakens: Focal length lengthens\n<ol class=\"wp-block-list\">\n<li>When turned fully, the beam diverges (becomes darker)<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<\/li>\n\n\n\n<li> Brightness: Coarse\/Fine adjustment selection\n<ol class=\"wp-block-list\">\n<li>[PANEL-L : BRIGHTNESS: CRS] While selected, the backlight turns on and the changing speed becomes x16.<\/li>\n<\/ol>\n<\/li>\n\n\n\n<li>Brightness confirmation\n<ol class=\"wp-block-list\">\n<li>Confirm visually on the screen<\/li>\n\n\n<li>[TC: TEM System Task Bar : SCREEN]<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n\n\n<h3 class=\"wp-block-heading\">Beam Centering<\/h3>\n\n\n<ol class=\"wp-block-list\">\n<li>[PANEL-R : STD FOCUS] Recall objective lens reference current\n<ol class=\"wp-block-list\">\n<li>Just in case<\/li>\n<\/ol>\n<\/li>\n\n\n\n<li>[PANEL-L : BRIGHTNESS] Converge the beam on the fluorescent screen.<\/li>\n\n\n\n<li>[PANEL-L : DEF\/STIG : BRIGHT TILT] Select bright field illumination mode<\/li>\n\n\n\n<li>[PANEL-L\/R: SHIFT-X\/Y] Move the beam to the center of the fluorescent screen with Beam Shift<\/li>\n<\/ol>\n\n\n<h3 class=\"wp-block-heading\">Condenser Lens Astigmatism Correction<\/h3>\n\n\n<ol class=\"wp-block-list\">\n<li>[PANEL-L : BRIGHTNESS] Converge the beam on the fluorescent screen.<\/li>\n\n\n\n<li>[PANEL-L : DEF\/STIG : COND STIG] Select condenser lens astigmatism adjustment mode<\/li>\n\n\n\n<li>[PANEL-L\/R: DEF\/STIG-X\/Y] (Cond STIG) Make the spot shape closer to a perfect circle.<\/li>\n<\/ol>\n\n\n<h3 class=\"wp-block-heading\">SPOT SIZE \/ \u03b1 Selection (TEM Mode)<\/h3>\n\n\n<ul class=\"wp-block-list\">\n<li>SPOT SIZE: Size of the electron beam when most converged\n<ul class=\"wp-block-list\">\n<li>Smaller is brighter<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li>\u03b1: Convergence angle when the electron beam is focused on the specimen. (Size of the parallel illumination area)<\/li>\n<\/ul>\n\n\n<ol class=\"wp-block-list\">\n<li>[PANEL-R : STD FOCUS] Recall objective lens reference current\n<ol class=\"wp-block-list\">\n<li>Just in case<\/li>\n<\/ol>\n<\/li>\n\n\n\n<li>[PANEL-L : PROBE CONTROL : SPOT SIZE] SPOT SIZE setting (1 to 5: Normal: 2)<\/li>\n\n\n\n<li>[PANEL-L : PROBE CONTROL : \u03b1SELECTOR] Convergence angle (illumination area) setting (1 to 9: Normal: 2)<\/li>\n\n\n\n<li>Perform <strong>Beam centering<\/strong> and <strong>Condenser lens astigmatism correction<\/strong> (if necessary)<\/li>\n<\/ol>\n\n\n<h3 class=\"wp-block-heading\">Gun Tilt Adjustment (Simple)<\/h3>\n\n\n<p class=\"has-background wp-block-paragraph\">&#x1f4a1; Make the electron beam emitted from the GUN incident straight onto the specimen along the optical axis.\nAssuming other optical systems are well adjusted.<\/p>\n\n\n<ol class=\"wp-block-list\">\n<li>[PANEL-R : Function : MAG1] <span><strong>To image observation mode<\/strong><\/span>\n<ol class=\"wp-block-list\">\n<li>[PANEL-L : APERTURE CONTROL : OL(OPEN)] Objective aperture: OPEN<\/li>\n\n\n<li>[HCA] High contrast objective aperture: OPEN<\/li>\n\n\n<li>[SA] Selected area aperture: OPEN<\/li>\n<\/ol>\n<\/li>\n\n\n\n<li>[PANEL-R : MAG\/CAM L]  <span><strong>Magnification setting<\/strong><\/span> (Normal: around x5000)<\/li>\n\n\n\n<li>[PANEL-R : STD FOCUS] Recall objective lens reference current\n<ol class=\"wp-block-list\">\n<li>Just in case<\/li>\n<\/ol>\n<\/li>\n\n\n\n<li><span><strong>Beam centering<\/strong><\/span>\n<ol class=\"wp-block-list\">\n<li>[PANEL-L : BRIGHTNESS] Converge the beam on the fluorescent screen.<\/li>\n\n\n<li>[PANEL-L : DEF\/STIG : BRIGHT TILT] Select bright field illumination mode<\/li>\n\n\n<li>[PANEL-L\/R: SHIFT-X\/Y] Center with Beam Shift<\/li>\n<\/ol>\n<\/li>\n\n\n\n<li>GUN TILT adjustment\n<ol class=\"wp-block-list\">\n<li>[TEM Center: Upper menu] Control \u2192 Alignment Panel (If not displayed)\n<ol class=\"wp-block-list\">\n<li>Alignment Panel (for JEM Administrators) will be displayed.<\/li>\n<\/ol>\n<\/li>\n\n\n<li>[TC : Alignment Panel : Deflector : GUN Align] Select GUN alignment mode<\/li>\n\n\n<li>[PANEL-L\/R: DEF\/STIG-X\/Y] Make the beam brightest with Beam Tilt.<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n\n\n<h3 class=\"wp-block-heading\">Condenser Lens Alignment<\/h3>\n\n\n<p class=\"has-background wp-block-paragraph\">&#x1f4a1; Reduce beam escape when changing SPOT SIZE.<\/p>\n\n\n<ol class=\"wp-block-list\">\n<li>Adjust GUN Tilt in advance. (If necessary)<\/li>\n\n\n\n<li>[PANEL-R : Function : MAG1] <span><strong>To image observation mode<\/strong><\/span>\n<ol class=\"wp-block-list\">\n<li>[PANEL-L : APERTURE CONTROL : OL(OPEN)] Objective aperture: OPEN<\/li>\n\n\n<li>[HCA] High contrast objective aperture: OPEN<\/li>\n\n\n<li>[SA] Selected area aperture: OPEN<\/li>\n<\/ol>\n<\/li>\n\n\n\n<li>[PANEL-R : MAG\/CAM L]  <span><strong>Magnification setting<\/strong><\/span> (Normal: around 50K)<\/li>\n\n\n\n<li>[PANEL-R : STD FOCUS] Recall objective lens reference current\n<ol class=\"wp-block-list\">\n<li>Just in case<\/li>\n<\/ol>\n<\/li>\n\n\n\n<li>[PANEL-L : BRIGHTNESS] Converge the beam on the fluorescent screen.<\/li>\n\n\n\n<li> Adjust at SPOT SIZE 1\n<ol class=\"wp-block-list\">\n<li>[PANEL-L : PROBE CONTROL : SPOT SIZE] Select 1<\/li>\n\n\n<li>[TEM Center: Upper menu] Control \u2192 Alignment Panel (If not displayed)\n<ol class=\"wp-block-list\">\n<li>Alignment Panel (for JEM Administrators) will be displayed.<\/li>\n<\/ol>\n<\/li>\n\n\n<li>[TC : Alignment Panel : Deflector : GUN Align] Select GUN alignment mode<\/li>\n\n\n<li>[PANEL-L\/R: SHIFT-X\/Y] Move the beam to the center of the fluorescent screen with Beam Shift<\/li>\n<\/ol>\n<\/li>\n\n\n\n<li> Adjust at SPOT SIZE 5\n<ol class=\"wp-block-list\">\n<li>[PANEL-L : PROBE CONTROL : SPOT SIZE] Select 5<\/li>\n\n\n<li>[PANEL-L : DEF\/STIG : BRIGHT TILT] or [TC : Alignment Panel : Deflector : Bright Tilt ] Select bright field illumination mode<\/li>\n\n\n<li>[PANEL-L\/R: SHIFT-X\/Y] Move the beam to the center of the fluorescent screen with Beam Shift<\/li>\n<\/ol>\n<\/li>\n\n\n\n<li>Repeat [Adjust at SPOT SIZE 1] and [Adjust at SPOT SIZE 5]<\/li>\n<\/ol>\n\n\n<h3 class=\"wp-block-heading\">Condenser Aperture Centering<\/h3>\n\n\n<p class=\"has-text-color wp-block-paragraph\">(Reference) JEM-2100plus: Aperture Operation: Condenser Aperture<\/p>\n\n\n<p class=\"has-background wp-block-paragraph\">&#x1f4a1; If the beam does not change concentrically (swings) when BRIGHTNESS is changed, the condenser aperture is misaligned from the optical axis, so adjust referring to the above.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><em>*This post was created by an AI agent.<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Note: This page was translated by AI from the Japanese original. In case of any discrepancy, the Japanese version shall prevail. Original post date (Japanese version): June 22, 2024 &#x1f4a1; This page is a quick manual for the JEM-2100plus. The contents are subject to change. Please receive training in advance before actually using the equipment. <a href=\"https:\/\/www.aem.imr.tohoku.ac.jp\/en\/jem-2100plus%ef%bc%9a%e7%85%a7%e5%b0%84%e7%b3%bb%e8%aa%bf%e6%95%b4\/\" class=\"more-link\">&#8230;<span class=\"screen-reader-text\">  JEM-2100plus: Illumination System Alignment<\/span><\/a><\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_locale":"en_US","_original_post":"https:\/\/www.aem.imr.tohoku.ac.jp\/?p=3203","footnotes":"","jetpack_post_was_ever_published":false},"categories":[1],"tags":[],"class_list":["post-5450","post","type-post","status-publish","format-standard","hentry","category-uncategorized","en-US"],"jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/posts\/5450","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/comments?post=5450"}],"version-history":[{"count":4,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/posts\/5450\/revisions"}],"predecessor-version":[{"id":5759,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/posts\/5450\/revisions\/5759"}],"wp:attachment":[{"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/media?parent=5450"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/categories?post=5450"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.aem.imr.tohoku.ac.jp\/wp-json\/wp\/v2\/tags?post=5450"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}