{"id":5466,"date":"2026-08-07T06:55:36","date_gmt":"2026-08-06T21:55:36","guid":{"rendered":"https:\/\/www.aem.imr.tohoku.ac.jp\/?p=5466"},"modified":"2026-08-07T06:55:36","modified_gmt":"2026-08-06T21:55:36","slug":"jem-2100plus-%e8%a9%a6%e6%96%99%e3%83%9b%e3%83%ab%e3%83%80%e6%8c%bf%e5%85%a5","status":"publish","type":"post","link":"https:\/\/www.aem.imr.tohoku.ac.jp\/en\/jem-2100plus-%e8%a9%a6%e6%96%99%e3%83%9b%e3%83%ab%e3%83%80%e6%8c%bf%e5%85%a5\/","title":{"rendered":"JEM-2100plus: Specimen Holder Insertion"},"content":{"rendered":"\n

Note: This page was translated by AI from the Japanese original. In case of any discrepancy, the Japanese version shall prevail.<\/em><\/p>\n\n\n\n

Original post date (Japanese version): June 22, 2024<\/em><\/p>\n\n\n\n

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  1. Pre-insertion Check (TEM Main Body)\n
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    1. Specimen holder is not inserted<\/li>\n\n\n
    2. [TEM Center: Upper menu] Control \u2192 Beam\n
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      • Display Beam Controller (for JEM Administrators)<\/li>\n\n\n
      • Check accelerating voltage and emission<\/strong><\/span><\/li>\n<\/ul>\n<\/li>\n\n\n
      • [TEM Center: Upper menu] View \u2192 Valve\/Vacuum Monitor\n
          \n
        • Display Valve\/Vacuum Monitor<\/li>\n\n\n
        • Check vacuum level<\/strong><\/span><\/li>\n<\/ul>\n<\/li>\n\n\n
        • [TEM Center: Upper menu] View \u2192 Condition Monitor\n
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          • Display Condition Monitor<\/li>\n\n\n
          • Check alarms<\/strong><\/span><\/li>\n<\/ul>\n<\/li>\n\n\n
          • [TEM Center: Upper menu] Control \u2192 Aperture\n
              \n
            • Display Aperture Panel Controller<\/li>\n\n\n
            • Retract the in-gap objective lens aperture<\/strong><\/span><\/li>\n\n\n
            • Select [OPEN] in Hole Diameter Select<\/li>\n<\/ul>\n<\/li>\n\n\n
            • [TEM Center: Left side TEM System Task Bar] Stage\n
                \n
              • Check stage position and tilt<\/strong><\/span><\/li>\n<\/ul>\n<\/li>\n<\/ol>\n<\/li>\n\n\n\n
              • Pre-insertion Check (Specimen\/Holder)\n
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                1. Specimen check\n
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                  1. The specimen is correctly fixed and does not move.<\/li>\n\n\n
                  2. No adhesion of sebum, dust, etc., near the specimen.<\/li>\n\n\n
                  3. Ion cleaning has been performed if necessary.<\/li>\n<\/ol>\n<\/li>\n\n\n
                  4. Holder check\n
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                    1. The tip of the holder is not damaged or deformed.<\/li>\n\n\n
                    2. No dust is attached to the O-ring. No scratches.<\/li>\n\n\n
                    3. (For 2-axis holder) Y tilt is 0.\n
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                      1. Check both the value on the TEM center and the actual stage tilt<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<\/li>\n\n\n\n
                      2. Holder Insertion\n
                          \n
                        1. Hold the holder handle and the front of the O-ring with both hands, align the guide pin, and insert it straight into the goniometer.\n
                            \n
                          1. Push it in until it stops, keeping your hand on it.<\/li>\n\n\n
                          2. At this position, the insertion detection switch operates. (It will be released if rotated, so do not move your hand)<\/li>\n<\/ol>\n<\/li>\n\n\n
                          3. Goniometer: Switch the PUMP\/AIR switch to PUMP.\n
                              \n
                            1. The specimen pre-chamber is evacuated.<\/li>\n\n\n
                            2. Wait until the green lamp lights up and PiG4 becomes EvacReady (green).<\/li>\n<\/ol>\n<\/li>\n\n\n
                            3. Insert the holder\n
                                \n
                              1. Turn the holder clockwise (slightly). (Until it stops)<\/li>\n\n\n
                              2. The holder is drawn in. (Slightly)<\/li>\n\n\n
                              3. Turn the holder clockwise (largely). (Until it stops)<\/li>\n\n\n
                              4. The holder is (largely) drawn in. (Do not let go until it stops)<\/li>\n\n\n
                              5. (For 2-axis holder) Connect the cable for Y-axis tilt.<\/li>\n<\/ol>\n\n\n

                                💡 In the above work<\/p>\n<\/li>\n\n\n

                              6. Select the holder\n
                                  \n
                                1. When the holder is inserted, the Specimen pane is displayed.\n
                                    \n
                                  • [TEM Center: Upper menu] Control \u2192 Specimen<\/li>\n<\/ul>\n<\/li>\n\n\n
                                  • Select the inserted holder from the Specimen Holder drop-down list.<\/li>\n\n\n
                                  • Click the [OK] button\n
                                      \n
                                    • Since the movement and tilt range of the stage differs for each holder, be sure to select the inserted holder.<\/li>\n<\/ul>\n<\/li>\n<\/ol>\n<\/li>\n\n\n
                                    • Wait for the vacuum to recover\n
                                        \n
                                      1. Penning Gauge 20 or less<\/li>\n\n\n
                                      2. SIP 10^-5Pa range<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n\n\n

                                        Manual Video (youtube channel)<\/h3>\n\n\n
                                        \n