{"id":5474,"date":"2026-08-07T06:55:36","date_gmt":"2026-08-06T21:55:36","guid":{"rendered":"https:\/\/www.aem.imr.tohoku.ac.jp\/?p=5474"},"modified":"2026-08-07T06:55:36","modified_gmt":"2026-08-06T21:55:36","slug":"jem-2100plus-%e8%a9%a6%e6%96%99%e3%82%bb%e3%83%83%e3%83%88%ef%bc%881%e8%bb%b8%e5%82%be%e6%96%9c%e3%83%9b%e3%83%ab%e3%83%80%ef%bc%89","status":"publish","type":"post","link":"https:\/\/www.aem.imr.tohoku.ac.jp\/en\/jem-2100plus-%e8%a9%a6%e6%96%99%e3%82%bb%e3%83%83%e3%83%88%ef%bc%881%e8%bb%b8%e5%82%be%e6%96%9c%e3%83%9b%e3%83%ab%e3%83%80%ef%bc%89\/","title":{"rendered":"JEM-2100plus: Specimen Mounting (Single Tilt Holder)"},"content":{"rendered":"\n

Note: This page was translated by AI from the Japanese original. In case of any discrepancy, the Japanese version shall prevail.<\/em><\/p>\n\n\n\n

Original post date (Japanese version): June 22, 2024<\/em><\/p>\n\n\n\n

💡 Handle the specimen and specimen holder carefully, as they are easily damaged.<\/p>\n\n\n

Specimen Mounting<\/h3>\n\n\n
    \n
  1. Preparation\n
      \n
    1. Place the specimen holder stand under a digital microscope (or stereomicroscope).<\/li>\n\n\n
    2. Gather tools in an easy-to-work position.\n
        \n
      1. Tweezers<\/li>\n\n\n
      2. Precision tweezers<\/li>\n\n\n
      3. Vacuum tweezers<\/li>\n\n\n
      4. Special screwdriver<\/li>\n\n\n
      5. Silicone mat (temporary specimen placement)<\/li>\n<\/ol>\n<\/li>\n\n\n
      6. Turn on the power of the digital microscope and focus on the specimen exchange stand.<\/li>\n<\/ol>\n<\/li>\n\n\n\n
      7. Specimen Preparation\n
          \n
        1. Specimen condition\n
            \n
          1. Must be sufficiently dry. (To avoid deteriorating the vacuum)<\/li>\n\n\n
          2. Must be securely fixed. (To prevent damage inside the column, and to prevent drift during observation)<\/li>\n\n\n
          3. Magnetization should be small. (To avoid being affected by the magnetic field of the objective lens)<\/li>\n\n\n
          4. The area around the observation target should be considered sufficiently thin (100nm or less).<\/li>\n<\/ol>\n<\/li>\n\n\n
          5. Take out the specimen and temporarily place it on the silicone mat.\n
              \n
            1. Make it easy to handle when mounting on the holder.\n
                \n
              1. It may be placed on weighing paper, etc.<\/li>\n\n\n
              2. You can take it out directly from the grid case. (When using precision tweezers)<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<\/li>\n\n\n\n
              3. Holder Preparation\n
                  \n
                1. Take out the holder to be used from the holder station. (Here, the 1-axis holder)\n
                    \n
                  1. Turn on the power of the holder station<\/li>\n\n\n
                  2. Valve Open\n
                      \n
                    1. Wait until it leaks to atmosphere.<\/li>\n<\/ol>\n<\/li>\n\n\n
                    2. Remove the holder.<\/li>\n<\/ol>\n<\/li>\n\n\n
                    3. Set the holder on the specimen holder stand. (Place the tip of the holder on the specimen exchange stand)<\/li>\n\n\n
                    4. Fix the holder with the screws of the holder support.<\/li>\n\n\n
                    5. Evacuate the holder station\n
                        \n
                      1. Insert dummy plug\/or holder<\/li>\n\n\n
                      2. Press the [EVAC] button\n
                          \n
                        1. Check TMP MONITOR\n
                            \n
                          1. ACC lights up<\/li>\n\n\n
                          2. Wait until RUNNING lights up.<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<\/li>\n\n\n
                          3. Valve Close<\/li>\n\n\n
                          4. Turn off the power of the holder station<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<\/li>\n\n\n\n
                          5. Specimen Loading\n
                              \n
                            1. Remove the specimen cartridge (holder tip)\n
                                \n
                              1. Release the fixation with the cartridge removal tool,<\/li>\n\n\n
                              2. Remove the cartridge with tweezers,<\/li>\n\n\n
                              3. Temporarily place it on the silicone mat.<\/li>\n<\/ol>\n<\/li>\n\n\n
                              4. Loosen the fixing screws with the special screwdriver (3 turns).\n

                                💡 Turning it too much will cause the screw to come off.\nIf not loosened enough, the plate holding spring will not move.<\/p>\n<\/li>\n\n\n

                              5. Rotate the specimen holding plate with precision tweezers.<\/li>\n\n\n
                              6. Place the specimen on the tip of the holder.\n