JEM-2100Plus: Image Observation (Basic)

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Note: This page was translated by AI from the Japanese original. In case of any discrepancy, the Japanese version shall prevail.

Original post date (Japanese version): June 22, 2024

💡 本ページはJEM-2100plusのクイックマニュアルです。 内容は変更される場合があります。 実際に装置を使用する場合は、事前にトレーニングを受けて下さい。

  1. [PANEL-R : Function : MAG1] 像観察モードへ
    1. [PANEL-L : APERTURE CONTROL :OL(OPEN)] Objective aperture: OPEN
    2. [HCA] High contrast objective aperture: OPEN
    3. [SA] Selected field aperture: OPEN
  2. [PANEL-R : STD FOCUS]　Objective lens reference current call

    💡 STF FOCUS can be done frequently.

  3. Probe settings
    1. [PANEL-L: PROBE CONTROL: TEM] TEM mode
    2. [PANEL-L : PROBE CONTROL : SPOT SIZE] SPOT SIZE setting (normal: 2)
    3. [PANEL-L : PROBE CONTROL : αSELECTOR] Convergence angle (irradiation range) setting (normal: 2)
    4. [CLA] Condenser aperture selection/centering (normal: 2)
  4. [PANEL-R : MAG/CAM L] Magnification setting(Usually: from about 10 to 50K)
  5. beam centering
    1. [PANEL-L: BRIGHTNESS] Focus the beam on the fluorescent screen. (crossover)
    2. [PANEL-L : DEF/STIG : BRIGHT TILT] 明視野照射モード選択
    3. [PANEL-L/R: SHIFT-X/Y] Centering with Beam Shift
  6. Condenser lens astigmatism adjustment
    1. [PANEL-L: DEF/STIG: COND STIG] Condenser lens astigmatism adjustment mode selection
    2. [PANEL-L/R: DEF/STIG-X/Y] (Cond STIG) Make the spot shape closer to a perfect circle.
  7. Brightness adjustment
    1. [PANEL-L: BRIGHTNESS] Brightness for easy observation: beam spread to the same extent as the screen
  8. Searching for field of view
    1. [TB] Move the observation target to the center of the field of view using the trackball
  9. Focusing (coarse)
    1. [PANEL-R : STD FOCUS]　基準電流呼び出し
    2. [PANEL-R: Z△▽] Sample height (Z) adjustment: Lowest contrast
  10. Objective lens astigmatism correction
    1. Medium double
      1. Observing the vicinity of the membrane hole (x20K ~)

        Center the beam and adjust the brightness for easy observation.

      2. [PANEL-L: DEF/STIG: OBJ STIG] Objective lens astigmatism adjustment mode selection
      3. [PANEL-R: OBJ FOCUS] Display Fresnel fringes with hyperfocus
      4. [PANEL-L/R: DEF/STIG-X/Y] Adjust the Fresnel fringe to be isotropic
    2. High magnification
      1. 非晶質領域（支持膜など）を観察（x100K～）

        Center the beam and adjust the brightness for easy observation.

      2. [PANEL-L: DEF/STIG: OBJ STIG] Objective lens astigmatism adjustment mode selection
      3. [PANEL-R : OBJ FOCUS] ＆[PANEL-L/R: DEF/STIG-X/Y]
        1. Confirm that the image flows due to hyperfocal <> underfocal
        2. Adjust to minimize image flow
  11. Insert objective aperture (if necessary)
    1. (Simple)
      1. [OLA/HCA] Objective aperture (in-gap orhigh contrast) insert
      2. [OLA/HCA] Centering of objective aperture
    2. （推奨）
      1. [SA] Inserting and centering the selected area diaphragm
      2. [PANEL-R : Function : DIFF] To diffraction mode: Electron diffraction display
      3. [OLA/HCA] Objective aperture (in gapor high contrast) and select transmitted wave.
      4. [PANEL-R : Function : MAG1] Return to image observation mode
      5. [SA] Pull out the selected area diaphragm
  12. Focusing (precision)
    1. [PANEL-R: OBJ FOCUS] Adjust to a focus that is easy to see near the positive focus
      1. 正焦点（ジャストフォーカス）：膜孔の輪郭のコントラスト最小
      2. Hyperfocal (overfocus): Focus clockwise from positive focus
      3. Underfocus: Move the focus from positive focus to counterclockwise.

      💡 Samples with low contrast will be unclear at positive focus, so set the focus to be slightly underfocused.

  13. (photograph)
    1. [Screen] Lower the magnification for CCD camera photography.
    2. [Screen] Adjust brightness
    3. Display the image with a CCD camera and adjust focus and brightness
    4. take a photo

*This post was created by an AI agent.