JEM-2100plus: Focus Alignment

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Note: This page was translated by AI from the Japanese original. In case of any discrepancy, the Japanese version shall prevail.

Original post date (Japanese version): June 22, 2024

TEM: Image Mode

💡 This page is a quick manual for the JEM-2100plus. The contents are subject to change. Please receive training in advance before actually using the equipment.

  1. Focusing (Coarse)
    1. [PANEL-R : STD FOCUS] Recall objective lens reference current
    2. [PANEL-R : Z△▽] Specimen height (Z) adjustment: Lower the contrast to the minimum
  2. Focusing (Fine)
    1. [PANEL-R : OBJ FOCUS] Adjust to an easy-to-see focus near the true focus
      1. True focus (Just focus): Minimum contrast of the outline of the hole in the film
      2. Overfocus: Turn Focus clockwise from true focus
      3. Underfocus: Turn Focus counterclockwise from true focus

    💡 Low-contrast specimens will be unclear at true focus, so set to a slight underfocus.

  3. Focusing using Wobbler
    1. Complete focusing (coarse) in advance.
    2. [PANEL-R : IMAGEWOBBLER : IMAGE X or Y] Turn image wobbler On
    3. Open the objective aperture. (Or set it to a large size)
    4. If out of focus, the image will appear double.
      1. [PANEL-R : OBJ FOCUS] Adjust so that the image overlaps as one

      💡 If the image does not overlap, correct the astigmatism of the objective lens

    5. [PANEL-R : IMAGEWOBBLER : IMAGE X or Y] Turn image wobbler Off

TEM: DIFF Mode

(Reference) JEM-2100plus: Electron Diffraction (Basics)

*This post was created by an AI agent.